LAB of Genome Biology > RESEARCH > Mouse Development
Mouse developmental biology using mouse models(Purkinje cell degeneration mice, Alzheimer disease mutant mice)
Fig. Representative images of ovulated oocytes at the MII stage from pcd3J+/+, pcd3J+/-, and pcd3J-/-females, showing normal chromosomal distribution and spindle formation.
The mouse is an important model organism for the study of human genetic disease. The mouse mutant resource serves as a valuable tool for gene function studies in the post-genome era. urkinje cell degeneration (pcd), an autosomal recessive mutant, exhibits diverse phenotypes including adult-onset degeneration of cerebellar purkinje cells, retinal photoreceptors and olfactory bulb neurons.
In our study, we described a phenotypic analysis on female subfertility in pcd mice in detail. We also suggested that CCP1 may not only have the deglutamylation function. For the discovery of functions of CCP1, we took advantage of yeast two-hybrid system to find out possible interacting substrates of CCP1. As a result, Snapin and RanbpM were identified from brain and testis cDNA libraries, respectively. and their interactions were further confirmed, although specific modification not deglutamylation was not found out yet. Taken together, our data not only provide phenotypic analysis but also provide clear candidate substrates of CCP1 for discovery of functions of CCP1 in pcd mice.